Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

This Anti-JAK1 Antibody is validated for use in IP, WB for the detection of JAK1.

Immunoprecipitation: Use at 5-10µl per 1-2mg lysate

Western blot: (1:1000-1:2000)

The amount of JAK1 in cell lines tends to be extremely low. If the signal is weak it is recommended that a RIPA cell lysate (1-2mg protein) be prepared and JAK1 be first concentrated by immunoprecipitation and then immunoblotted. A degradation product of JAK1 (~110kDa) is also detected by the antiserum in immunoblots.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to 21 amino acids of human/mouse/rat JAK1

(KTLIEKERFYESRCRPVTPS-C)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Serum. Whole rabbit antiserum containing sodium azide.

Specificity

Recognizes JAK1 (Janus Kinase 1). The members of the JAK family of cytoplasmic protein tyrosine kinases physically associate with ligand-bound receptors. It is this association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.